This paper with the connotation of urbanization establishes an evaluation index system to reflect urbanization development level based on population, economy, livelihood and regional environment, and evaluates comprehensively the urbanization development level among 13 cities which are classified by means of clustering analysis, aiming at identifying advantages and disadvantages in these cities. Meanwhile, via GIS, the urbanization disparity is reflected on spatial locations in order to make a policy on improving the regional urbanization level within Jiangsu Province.